2,43
1,65
1,98
0,73
1,88
1,81
2,43
2.2 Standardstoffer anvendt i kalibreringskurven for relativ molekylmassefordeling: insulin, mycopeptider, glycin-glycin-tyrosin-arginin, glycin-glycin-glycin
3 Instrument og udstyr
23.2
21.4
22.2
16.1
22.3
20,8
23,9
27,5
Samlet set er andelen af aminosyrer i Sustars produkter højere end i Zinpros produkter.
Del 8 Virkninger af brug
Effekter af forskellige kilder til spormineraler på produktionsydelse og ægkvalitet hos æglæggende høner i den sene æglægningsperiode
Produktionsproces
Målrettet keleringsteknologi
Forskydningsemulgeringsteknologi
Tryksprøjte- og tørringsteknologi
Køle- og affugtningsteknologi
Avanceret miljøkontrolteknologi
Bilag A: Metoder til bestemmelse af relativ molekylmassefordeling af peptider
Vedtagelse af standard: GB/T 22492-2008
1 Testprincip:
Det blev bestemt ved hjælp af højtydende gelfiltreringskromatografi. Det vil sige, ved at bruge porøst fyldstof som stationær fase, baseret på forskellen i den relative molekylmassestørrelse af prøvekomponenterne til separation, detekteret ved peptidbindingen med den ultraviolette absorptionsbølgelængde på 220 nm, ved hjælp af den dedikerede databehandlingssoftware til bestemmelse af relativ molekylmassefordeling ved gelfiltreringskromatografi (dvs. GPC-softwaren), blev kromatogrammerne og deres data behandlet og beregnet for at få størrelsen af den relative molekylmasse af sojabønnepeptidet og fordelingsområdet.
2. Reagenser
Forsøgsvandet skal opfylde specifikationen for sekundært vand i GB/T6682, og brugen af reagenser skal være analytisk rene, bortset fra særlige bestemmelser.
2.1 Reagenser omfatter acetonitril (kromatografisk ren), trifluoreddikesyre (kromatografisk ren),
2.2 Standardstoffer anvendt i kalibreringskurven for relativ molekylmassefordeling: insulin, mycopeptider, glycin-glycin-tyrosin-arginin, glycin-glycin-glycin
3 Instrument og udstyr
3.1 Højtydende væskekromatograf (HPLC): en kromatografisk arbejdsstation eller integrator med en UV-detektor og GPC-databehandlingssoftware.
3.2 Mobil fase vakuumfiltrerings- og afgasningsenhed.
3.3 Elektronisk vægt: gradueret værdi 0,000 1 g.
4 Betjeningstrin
4.1 Kromatografiske betingelser og systemtilpasningsforsøg (referencebetingelser)
- 4.1.1 Kromatografisk kolonne: TSKgelG2000swxl300 mm × 7,8 mm (indre diameter) eller andre gelkolonner af samme type med lignende ydeevne, der er egnede til bestemmelse af proteiner og peptider.
- 4.1.2 Mobil fase: Acetonitril + vand + trifluoreddikesyre = 20 + 80 + 0,1.
- 4.1.3 Detektionsbølgelængde: 220 nm.
- 4.1.4 Strømningshastighed: 0,5 ml/min.
- 4.1.5 Detektionstid: 30 min.
- 4.1.6 Prøveindsprøjtningsvolumen: 20 μL.
- 4.1.7 Kolonnetemperatur: stuetemperatur.
- 4.1.8 For at det kromatografiske system skulle opfylde detektionskravene, blev det fastsat, at gelkromatografisøjlens effektivitet, dvs. det teoretiske antal plader (N), under ovenstående kromatografiske betingelser ikke skulle være mindre end 10000 beregnet på basis af toppene af tripeptidstandarden (glycin-glycin-glycin).
- 4.2 Fremstilling af standardkurver for relativ molekylmasse
- Ovennævnte forskellige peptidstandardopløsninger med relativ molekylmasse og en massekoncentration på 1 mg/ml blev fremstillet ved mobil fasematchning, blandet i en bestemt mængdeforhold og derefter filtreret gennem en organisk fasemembran med en porestørrelse på 0,2 μm~0,5 μm og injiceret i prøven, hvorefter standardernes kromatogrammer blev opnået. Kalibreringskurver for relativ molekylmasse og deres ligninger blev opnået ved at plotte logaritmen af den relative molekylmasse mod retentionstid eller ved lineær regression.
4.3 Prøvebehandling
Vej nøjagtigt 10 mg prøve i en 10 ml målekolbe, tilsæt lidt mobil fase, ryst med ultralyd i 10 minutter, så prøven er fuldstændigt opløst og blandet, fortynd med mobil fase til vægten, og filtrer derefter gennem en organisk fasemembran med en porestørrelse på 0,2 μm~0,5 μm, og filtratet blev analyseret i henhold til de kromatografiske betingelser i A.4.1.
- 5. Beregning af relativ molekylmassefordeling
- Efter analyse af prøveopløsningen fremstillet i 4.3 under de kromatografiske betingelser i 4.1, kan prøvens relative molekylmasse og dens fordelingsområde bestemmes ved at indsætte prøvens kromatografiske data i kalibreringskurven 4.2 med GPC-databehandlingssoftware. Fordelingen af de relative molekylmasser af de forskellige peptider kan beregnes ved hjælp af peak-arealnormaliseringsmetoden i henhold til formlen: X=A/A total×100
- I formlen: X - Massefraktionen af et relativ molekylmassepeptid i det samlede peptid i prøven, %;
- A - Topareal af et peptid med relativ molekylmasse;
- Total A - summen af toparealerne for hvert relative molekylmassepeptid, beregnet med én decimal.
- 6 Repeterbarhed
- Den absolutte forskel mellem to uafhængige bestemmelser opnået under repeterbarhedsbetingelser må ikke overstige 15 % af det aritmetiske gennemsnit af de to bestemmelser.
- Bilag B: Metoder til bestemmelse af frie aminosyrer
- Vedtagelse af standard: Q/320205 KAVN05-2016
- 1.2 Reagenser og materialer
- Iseddikesyre: analytisk ren
- Perchlorsyre: 0,0500 mol/L
- Indikator: 0,1% krystalviolet indikator (iseddikesyre)
- 2. Bestemmelse af frie aminosyrer
Prøverne blev tørret ved 80 °C i 1 time.
Placer prøven i en tør beholder for at afkøle den naturligt til stuetemperatur eller til en brugbar temperatur.Vej ca. 0,1 g prøve (med en nøjagtighed på 0,001 g) af i en 250 ml tør konisk kolbe.Gå hurtigt videre til næste trin for at undgå, at prøven absorberer omgivende fugt.Tilsæt 25 ml iseddikesyre og bland godt i højst 5 minutter.Tilsæt 2 dråber krystalviolet indikatorTitrer med 0,0500 mol/L (±0,001) standardtitreringsopløsning af perchlorsyre, indtil opløsningen skifter farve fra lilla til slutpunktet.
Registrer den forbrugte mængde standardopløsning.
- Udfør blindprøven på samme tid.
- 3. Beregning og resultater
- Indholdet af frie aminosyrer X i reagenset udtrykkes som en massefraktion (%) og beregnes efter formlen: X = C × (V1-V0) × 0,1445/M × 100%, i formlen:
- C - Koncentration af standard perchlorsyreopløsning i mol pr. liter (mol/L)
- V1 - Volumen anvendt til titrering af prøver med standard perchlorsyreopløsning, i milliliter (ml).
- Vo - Volumen anvendt til titreringsblindprøve med standard perchlorsyreopløsning, i milliliter (ml);
M - Prøvens masse i gram (g).
| 0,1445: Gennemsnitlig masse af aminosyrer svarende til 1,00 mL standard perchlorsyreopløsning [c(HClO4) = 1,000 mol/L]. | 4.2.3 Standardtitreringsopløsning af ceriumsulfat: koncentration c [Ce(SO4)2] = 0,1 mol/L, fremstillet i henhold til GB/T601. | |
| Vedtagelse af standarder: Q/70920556 71-2024 | 1. Bestemmelseprincip (Fe som eksempel) | Aminosyrejernkomplekser har meget lav opløselighed i vandfri ethanol, og frie metalioner er opløselige i vandfri ethanol. Forskellen i opløselighed mellem de to i vandfri ethanol blev anvendt til at bestemme chelateringshastigheden af aminosyrejernkomplekser. |
| I formlen: V1 - volumen af ceriumsulfatstandardopløsning forbrugt til titrering af testopløsningen, ml; | Vandfri ethanol; resten er den samme som i punkt 4.5.2 i GB/T 27983-2011. | 3. Analysetrin |
| Udfør to forsøg parallelt. Vej 0,1 g af prøven, tørret ved 103 ± 2 ℃ i 1 time, med en nøjagtighed på 0,0001 g, tilsæt 100 ml vandfri ethanol for at opløse den, filtrer, filtrer resten, og skyl den med 100 ml vandfri ethanol mindst tre gange, og overfør derefter resten til en 250 ml konisk kolbe, tilsæt 10 ml svovlsyreopløsning i henhold til klausul 4.5.3 i GB/T27983-2011, og udfør derefter følgende trin i henhold til klausul 4.5.3 “Opvarm til opløsning og lad det køle af” i GB/T27983-2011. Udfør blindprøven samtidig. | 4. Bestemmelse af det samlede jernindhold | 4.1 Bestemmelsesprincippet er det samme som i punkt 4.4.1 i GB/T 21996-2008. |
4.2. Reagenser og opløsninger
| 4.2.1 Blandet syre: Tilsæt 150 ml svovlsyre og 150 ml fosforsyre til 700 ml vand og bland godt. | 4.2.2 Indikatoropløsning af natriumdiphenylaminsulfonat: 5 g/L, fremstillet i henhold til GB/T603. | 4.2.3 Standardtitreringsopløsning af ceriumsulfat: koncentration c [Ce(SO4)2] = 0,1 mol/L, fremstillet i henhold til GB/T601. | |
| 4.3 Analysetrin | Udfør to forsøg parallelt. Vej 0,1 g prøve med en nøjagtighed på 020001 g, anbring den i en 250 ml konisk kolbe, tilsæt 10 ml blandet syre. Efter opløsning tilsættes 30 ml vand og 4 dråber natriumdianilinsulfonatindikatoropløsning, og udfør derefter følgende trin i henhold til klausul 4.4.2 i GB/T21996-2008. Udfør blindprøven samtidig. | 4.4 Repræsentation af resultater | Det samlede jernindhold X1 i aminosyrejernkomplekserne udtrykt som massefraktion af jern, værdien udtrykt i %, blev beregnet i henhold til formel (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - ceriumsulfatstandardopløsning forbrugt til titrering af blindopløsning, ml; | V0 - ceriumsulfatstandardopløsning forbrugt til titrering af blindopløsning, ml; | C - Faktisk koncentration af ceriumsulfatstandardopløsning, mol/L5. Beregning af jernindhold i chelaterJernindholdet X2 i chelatet udtrykt som massefraktionen af jern, værdien udtrykt i %, blev beregnet efter formlen: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| I formlen: V1 - volumen af ceriumsulfatstandardopløsning forbrugt til titrering af testopløsningen, ml; | V2 - ceriumsulfatstandardopløsning forbrugt til titrering af blindopløsning, ml;nom1 - Prøvens masse, g. Tag det aritmetiske gennemsnit af de parallelle bestemmelsesresultater som bestemmelsesresultater, og den absolutte forskel mellem de parallelle bestemmelsesresultater er ikke mere end 0,3 %. | 0,05585 - masse af jern(II) udtrykt i gram svarende til 1,00 ml ceriumsulfatstandardopløsning C[Ce(SO4)2.4H20] = 1,000 mol/L.nom1 - Prøvens masse, g. Tag det aritmetiske gennemsnit af de parallelle bestemmelsesresultater som bestemmelsesresultater, og den absolutte forskel mellem de parallelle bestemmelsesresultater er ikke mere end 0,3 %. | 6. Beregning af keleringshastighedChelateringshastighed X3, værdien udtrykt i %, X3 = X2/X1 × 100Bilag C: Metoder til bestemmelse af Zinpros chelateringshastighed |
Vedtagelse af standard: Q/320205 KAVNO7-2016
1. Reagenser og materialer
a) Iseddikesyre: analytisk ren; b) Perchlorsyre: 0,0500 mol/L; c) Indikator: 0,1% krystalvioletindikator (iseddikesyre)
2. Bestemmelse af frie aminosyrer
2.1 Prøverne blev tørret ved 80 °C i 1 time.
2.2 Anbring prøven i en tør beholder for at afkøle den naturligt til stuetemperatur eller til en brugbar temperatur.
2.3 Vej ca. 0,1 g prøve (nøjagtigt til 0,001 g) i en 250 ml tør konisk kolbe.
2.4 Gå hurtigt videre til næste trin for at undgå, at prøven absorberer omgivende fugt.
2.5 Tilsæt 25 ml iseddikesyre og bland godt i højst 5 minutter.
2.6 Tilsæt 2 dråber krystalvioletindikator.
2.7 Titrer med 0,0500 mol/L (±0,001) standardtitreringsopløsning af perchlorsyre, indtil opløsningen skifter farve fra lilla til grøn i 15 sekunder uden at skifte farve som slutpunkt.
2.8 Registrer den forbrugte mængde standardopløsning.
2.9 Udfør blindprøven samtidig.
- 3. Beregning og resultater
- Catalansk
- Physicochemical parameters
V1 - Volumen anvendt til titrering af prøver med standard perchlorsyreopløsning, i milliliter (ml).
Vo - Volumen anvendt til titreringsblindprøve med standard perchlorsyreopløsning, i milliliter (ml);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
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Produkter
Uorganiske spormineraler
- Organiske spormineraler
- Swahili
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Firmaprofil
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Klik for forespørgsel | © Ophavsret - 2010-2025: Alle rettigheder forbeholdes. | Sitemap TOP SØGNING Telefon |
| Tlf. | 86-18880477902 | Javanesisk | E-mail |
| 8618880477902 | kinesisk | Fransk | |
| Bird | kinesisk | Fransk | Tysk Spansk |
| Aquatic animals | Japansk | Koreansk | Arabisk Græsk |
| Tyrkisk | Italiensk | ||
| Ruminant animal g/head day | January 0.75 | Indonesisk Afrikaans Svensk |
Polere
- Baskisk
- Catalansk
- Physicochemical parameters
Hindi
Laotisk
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgarsk
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Kroatisk
Hollandsk
| Application object | Urdu Vietnamesisk | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haitiansk | Hausa | Kinyarwanda Hmong Ungarsk |
| Piglets and fattening pigs | Igbo | Javanesisk | Kannada Khmer Kurdisk |
| Kirgisisk | Latin | ||
| Bird | 300～400 | 45～60 | Makedonsk Malayisk Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norsk
- Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbisk
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tadsjikisk
Tamil
Telugu
Thailandsk
| Application object | Urdu Vietnamesisk | Content in full-value feed (mg/kg) | Efficacy |
| jiddisch | Yoruba | Zulu | Kinyarwanda Oriya Turkmensk |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1,52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
